Bitflow frame grabbers do not support multiple ROIs or changing the ROI while the camera is acquiring. Users may able to just set the camera to the largest ROI and window down to the smaller ones as needed. In addition, for line scan camera, the image is acquired line by line so it is impossible to crop the image into different ROI during acquisition process. Users will need to write a post-image processing function to crop the image into different ROI after the image was captured .